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http://krishi.icar.gov.in/jspui/handle/123456789/7385| Title: | Detection of beef adulteration by mitochondrial D-loop based species-specific polymerase chain reaction. |
| Other Titles: | Not Available |
| Authors: | Nagappa K, Girish P.S, Kumar D and Singh S.P |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Meat, Hyderabad |
| Published/ Complete Date: | 2017-12-31 |
| Project Code: | Not Available |
| Keywords: | Beef, Adulteration, DNA, PCR, D-loop |
| Publisher: | Taylor & Francis Online |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A species-specific polymerase chain reaction (PCR) was developed for the identification of beef that is cattle (Bos indicus) meat by targeting mitochondrial D-loop region. A pair of beef-specific primers was designed to amplify conserved region of D-loop and possibility of cross-amplification was ruled by taking into account other species comprising of mammals, birds, rodents and fish. The amplified PCR product of 381 bp was evident in raw (n = 20), cooked (60, 80 and 100°C), autoclaved (121°C) and micro-oven processed beef samples. Sensitivity of PCR assay was at 0.1% for detection of beef adulteration and the limit of detection (LOD) of beef DNA was 0.1 picograms (pg). Newly developed beef-specific PCR assay was rapid and sensitive and hence could be applied for the detection of adulteration of meat. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | International Journal of Food Properties |
| NAAS Rating: | 7.81 |
| Volume No.: | 20(52) |
| Page Number: | 2264-2271 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1080/10942912.2017.1369103 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7385 |
| Appears in Collections: | AS-NRCMeat-Publication |
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