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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/7390
Title: | Molecular approaches in species identification of Sarcocysts in Indian buffalo meat. |
Other Titles: | Not Available |
Authors: | Ramakrishna C, Vaithiyanathan S, Muthukumar M, Chatlod L.R, Lavanya P and Kulkarni V.V |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Research Centre on Meat, Hyderabad |
Published/ Complete Date: | 2018-07-31 |
Project Code: | Not Available |
Keywords: | Buffalo, Identification, Meat, PCR, RFLP, Sarcocysts |
Publisher: | MRI Publications |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | DNA was extracted from sarcocysts (visible on naked eye examination) collected from 168 buffaloes belonging to Mumbai (60), Hyderabad (54) and Kolkata (54) cities of India. They were subjected to PCR assay using 18S rRNA gene primer. All the PCR amplicons of about 900 bp were subjected to restriction enzyme digestion with four different restriction enzymes (BslI, DraI, FokI and RsaI). PCR amplicons showed two different patterns (Pattern A and Pattern B) on RFLP. Twenty one PCR products from Pattern A and one PCR product from Pattern B were subjected to DNA sequencing. S. fusiformis and S. taeniata were identified from Pattern A and S. buffalonis was identified from Pattern B on sequencing. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Animal Research |
NAAS Rating: | 6.4 |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://www.myresearchjournals.com/index.php/IJAR/article/view/11657 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7390 |
Appears in Collections: | AS-NRCMeat-Publication |
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