Mango intra-varietal hybrid confirmation through SPAR markers

Abstract

Genetic studies in mango can increase pace of precision breeding due to development of true mapping populations endorsed by molecular markers. Concerns over the suitability of single dominant marker system for determining parentage have arisen due to appearance of nonparental bands in offspring of known pedigrees as well as heterozygosity at the marker loci. Twenty-one mango (Mangifera indica L.) hybrid progenies developed from inravarietal crossing could be established as true hybrids using RAPD, ISSR and DAMD markers. UPGMA tree showed clustering of the hybrids with the male parents i.e. Vanraj and Janardan Pasand respectively. Amrapali being common parent is equidistant from all hybrids and is isolated as a separate shoot confirming the pedigree. This may be attributed to the bearing habits as Amrapali is a regular bearer and late maturating variety, while Vanraj and Janardan Pasand are biennial bearers and peel colour donors. The results of the present study indicated that three markers together serve as a candidate molecular tool to correlate parent-hybrid relationship.