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http://krishi.icar.gov.in/jspui/handle/123456789/77080| Title: | Development of a liquid-phase blocking ELISA based on foot-and-mouth disease virus empty capsid antigen for seromonitoring vaccinated animals. |
| Other Titles: | Not Available |
| Authors: | S H Basagoudanavar M Hosamani R P Tamil Selvan B P Sreenivasa P Saravanan B K Chandrasekhar Sagar R Venkataramanan |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2012-12-16 |
| Project Code: | Not Available |
| Keywords: | foot-and-mouth disease liquid-phase blocking ELISA detection of antibodies |
| Publisher: | Archives of virology |
| Citation: | Not Available |
| Abstract/Description: | In foot-and-mouth disease (FMD) control programme, liquid-phase blocking ELISA (LPBE) is widely used to assay vaccine-induced seroconversion. Currently, the assay utilizes inactivated FMD virus antigen for the detection of antibodies in serum samples. To develop a non-infectious substitute for the antigen in LPBE, we expressed the structural polypeptide of FMDV (serotype A) using a baculovirus expression system, and show that inclusion of viral 3C with reduced protease activity resulted in a higher yield of structural proteins. Structural proteins expressed in insect cells assembled into empty virus-like particles (VLPs) and showed antigenicity comparable to chemically inactivated FMDV. Screening of serum samples from FMD-vaccinated cattle showed that the test performance of VLP-LPBE had a correlation of 0.89 with conventional inactivated virus antigen LPBE. The VLP-LPBE developed here demonstrates the diagnostic application of recombinant FMDV VLPs in monitoring seroconversion following FMD vaccination. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Archives of virology |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 8.69 |
| Impact Factor: | 2.68 |
| Volume No.: | 158 |
| Page Number: | 993-1001 |
| Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute bengaluru |
| Source, DOI or any other URL: | 10.1007/s00705-012-1567-5 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77080 |
| Appears in Collections: | AS-IVRI-Publication |
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