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http://krishi.icar.gov.in/jspui/handle/123456789/77212| Title: | Development and comparative evaluation of loop mediated isothermal amplification (LAMP) assay for simple visual detection of orf virus in sheep and goats. |
| Authors: | Venkatesan G, Bhanuprakash V, Balamurugan V. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR:Indian Veterinary Research Institute |
| Published/ Complete Date: | 2015-06-01 |
| Keywords: | Diagnosis; HNB dye; LAMP assay; Orf virus; SYBR green I |
| Publisher: | Elsevier |
| Abstract/Description: | A loop-mediated isothermal amplification (LAMP) assay targeting DNA Pol gene was optimized and evaluated for the rapid detection of orf virus in clinical samples. The LAMP assay was found to be specific and sensitive. The detection rate of LAMP (89.3%) was better than PCR (67.9%) and comparable to real-time PCR (91.1%) in clinical samples by gel electrophoresis and visual detection methods. This LAMP assay is simple and does not rely upon any special equipment and could be employed in clinical diagnosis and epidemiological survey of orf infection. |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Language: | English |
| Name of Journal: | Molecular and Cellular probes |
| Journal Type: | Included in NAAS Journal list |
| NAAS Rating: | 9.29 |
| Impact Factor: | 3.285 |
| Volume No.: | 29(3) |
| Page Number: | 193-195 |
| Name of the Division/Regional Station: | ICAR-IVRI Campus, Hebbal, Bengaluru 560024, Karnataka |
| Source, DOI or any other URL: | 10.1016/j.mcp.2015.03.006 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77212 |
| Appears in Collections: | AS-IVRI-Publication |
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