KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77249| Title: | Production and characterization of Monoclonal antibodies to bluetongue virus. |
| Authors: | Bhanuprakash V, Hosamani M, Balamurugan V, Gandhale PN, Venkatesan G, Singh RK. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR:Indian Veterinary Research Institute |
| Published/ Complete Date: | 2011-02-01 |
| Keywords: | Monoclonal antibodies, Bluetongue Virus, production, Characterization |
| Publisher: | Elsevier |
| Abstract/Description: | In the present study, a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein, titres, isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones, a majority of them (n = 18) belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA, the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However, this clone showed a variable percent of inhibition ranging from16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones, only 4A10 was found to have a possible diagnostic application. |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Language: | English |
| Name of Journal: | Virologica Sinica |
| Journal Type: | Included in NAAS Journal list |
| NAAS Rating: | 12.95 |
| Impact Factor: | 6.947 |
| Volume No.: | 26(1) |
| Page Number: | 8-18 |
| Name of the Division/Regional Station: | ICAR-IVRI Campus, Hebbal, Bengaluru 560024, Karnataka |
| Source, DOI or any other URL: | 10.1007/s12250-011-3171-8 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77249 |
| Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.