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http://krishi.icar.gov.in/jspui/handle/123456789/77700| Title: | Genetic identity between Bhadawari and Murrah breeds of Indian Buffaloes (Bubalus bubalis) using RAPD-PCR |
| Other Titles: | Not Available |
| Authors: | Saifi, H. W. Bhushan, B. Kumar S Kumar P Patra B N Sharma A |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2004-01-01 |
| Project Code: | Not Available |
| Keywords: | Bhadawari and Murrah Buffaloes, Band Frequency, Genetic Identity Index, Mean Average Percentage Difference, RAPDPCR |
| Publisher: | Animal Bioscience |
| Citation: | Saifi, H. W., Bhushan, B., Kumar, S., Kumar, P., Patra, B. N., & Sharma, A. (2004). Genetic identity between Bhadawari and Murrah breeds of Indian buffaloes (Bubalus bubalis) using RAPD-PCR. Asian-Australasian Journal of Animal Sciences, 17(5), 603-607. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out with a battery of 11 random decamer primers to study band frequency (BF), genetic identity index (I) and mean average percentage difference (MAPD) between Bhadawari and Murrah breeds of buffalo. The primers OPA04 and BG15 resolved a band of 460 bp, which was present only in animals of Bhadawari breed. Whereas, the primers OPA14, BG27 and BG28 produced Murrah specific fragments of sizes 730 bp and 1,230 bp, respectively. The estimate of genetic identity index was highest (0.845) with the primer OPA01 and the lowest (0.479) with the primer BG27. The genetic identity index pooled over the primers was 0.596±0.037 between these two breeds. The highest MAPD estimate (53.9) between the two breeds was obtained with the primer BG27 and the lowest (14.3) with the primer OPA01. It might be concluded that the genetic identity index between these two breeds calculated on the basis of BF showed moderate level of genetic identity with the primers employed. MAPD calculated on the basis of uncommon bands also demonstrated lower to medium level of genetic difference between Bhadawari and Murrah breeds of buffalo. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Division of Animal Genetics |
| Source, DOI or any other URL: | doi.org/10.5713/ajas.2004.603 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77700 |
| Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Saifi et al. (2004).pdf | 69.2 kB | Adobe PDF | View/Open |
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