KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77701| Title: | Development of a monoclonal antibody based Competitive ELISA for the detection of Rinderpest antibodies |
| Authors: | R P Singh B P Sreenivasa P Dhar R N Roy S K Bandyopadhyay |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2000-07-10 |
| Project Code: | Not Available |
| Keywords: | competitive enzyme-linked immunosorbent assay monoclonal antibody rinderpest |
| Publisher: | Revue scientifique et technique (International Office of Epizootics) |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A competitive enzyme-linked immunosorbent assay has been standardised for the detection of antibodies to rinderpest virus in sera from cattle, sheep and goats. The test uses a neutralising monoclonal antibody (MAb) directed against the haemagglutinin protein of rinderpest virus. The test is specific for rinderpest, as it failed to detect antibodies to peste des petits ruminants virus in convalescent goat sera. A 45% inhibition of the binding of the MAb to the antigen was used as the cut-off point for deciding the rinderpest status of the test samples. The specificity and sensitivity of the test and the stability of the test reagents were determined and compared to the results obtained using a commercial kit with approximately 1,200 serum samples from cattle, sheep and goats in India. The current test compared very well with the commercial kit. The test is expected to be extremely useful for sero-monitoring and sero-surveillance of rinderpest in countries which are actively pursuing a rinderpest eradication programme. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Revue scientifique et technique (International Office of Epizootics) |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 6.84 |
| Impact Factor: | 0.87 |
| Volume No.: | 19 (3) |
| Page Number: | 754-763 |
| Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute, mukteshwar |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.vetmic.2003.07.007 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77701 |
| Appears in Collections: | AS-IVRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.