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http://krishi.icar.gov.in/jspui/handle/123456789/81910| Title: | Development of CD163 receptor-based enzyme-linked immunosorbent assay for diagnosis of porcine reproductive and respiratory syndrome virus |
| Other Titles: | Not Available |
| Authors: | Rajib Deb, Ajay Kumar Yadav, Gyanendra Singh Sengar, Joyshikh Sonowal, D. Lalita, Seema Rani Pegu, Indra Singh, Ningthoukhongjam Linda, Pranab Jyoti Das, Satish Kumar, Prasanna Pal, Souvik Paul, Swaraj Rajkhowa & Vivek Kumar Gupta |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Pig |
| Published/ Complete Date: | 2022-10-15 |
| Project Code: | Not Available |
| Keywords: | receptor; enzyme; porcine |
| Publisher: | Not Available |
| Citation: | Deb, R., Yadav, A. K., Sengar, G. S., Sonowal, J., Lalita, D., Pegu, S. R., ... & Gupta, V. K. (2022). Development of CD163 receptor-based enzyme-linked immunosorbent assay for diagnosis of porcine reproductive and respiratory syndrome virus. 3 Biotech, 12(11), 325. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Porcine reproductive and respiratory syndrome (PRRS) is an important economical disease in the global swine industry. The accurate detection of the PRRS virus (PRRSV) antigen is essential for the disease control and prevention programme. In this study, an indirect enzyme-linked immunosorbent test (PRRSVCD163-iELISA) was developed for the detection of the PRRSV antigen in samples of post-mortem swine tissue using the recombinant pig CD163 receptor protein as the capture ligand. The test was found to be specific for PRRSV, with no cross-reactions with other prevalent pig viral pathogens. The assay was validated by testing 217 post-mortem porcine tissue samples and the results were found to be satisfactory with a relative accuracy of 88.88%. Our assay is also quite precise, with intra- and inter-assay CVs of 6% and 10%, respectively. These findings imply that the PRRSVCD163-iELISA developed is capable of detecting the PRRSV antigen in swine post-mortem tissue samples. This research showed that porcine CD163, the PRRSV cellular receptor, can be exploited to build a diagnostic technique for the detection of PRRSV antigen. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | 3 Biotech |
| Journal Type: | NAAS Listed |
| NAAS Rating: | 8.80 |
| Impact Factor: | 3.1 |
| Volume No.: | 12 |
| Page Number: | 325 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s13205-022-03376-z |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/81910 |
| Appears in Collections: | AS-NRCP-Publication |
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