Physiological and immune responses to long road transportation in Andaman local pigs

Abstract

Transportation of farm animals is a routine management practice. During transportation, animals experience physiological and psychological stress which has negative consequences on health and welfare of animals. Effect of transportation on pig health and welfare in island ecosystem is not properly known. Here, we report the effect of transportation stress under island ecosystem on serum biochemical parameters, lipid profile, stress biomarkers, and immune parameters in Andaman local pig. Twelve pigs were transported from North and Middle Andaman to South Andaman via road in which two small sea channels have to be crossed in ferry with vehicle on board. Transportation triggered increase in serum levels of total protein and albumin. Elevated levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and creatine kinase in transported pigs indicated muscular exercise or impaired liver function. Transportation induced dyslipidemia indicated by elevated levels of total cholesterol, low-density lipoprotein cholesterol, and triglycerides and a decreased level of high-density lipoprotein cholesterol. High level of serum total nitric oxide and serum MDA as well as low antioxidant capacity in transported pigs indicated oxidative stress induction. Transportation resulted in activation of stress response and increased levels of serum cortisol and heat shock proteins were recorded in transported pigs. Moreover, increased concentration of pro-inflammatory cytokines (IL-2, IL-6, IL-12, IL-1β, and IFN-γ) and decreased concentration of anti-inflammatory cytokine IL-4 were detected in transported pigs. The results of the study are helpful for better understanding of transportation stress in pigs under island ecosystem.