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http://krishi.icar.gov.in/jspui/handle/123456789/8992| Title: | Development of an enzyme immunoassay using recombinant invariant surface glycoprotein (rISG) 75 for serodiagnosis of bovine trypanosomosis |
| Other Titles: | Not Available |
| Authors: | Rudramurthy GR Sengupta PP Metilda B Balamurugan V Prabhudas K Rahman H |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2015-01-01 |
| Project Code: | Not Available |
| Keywords: | Cattle Extracellular domain Parasitaemia Surra Trypanosoma evansi |
| Publisher: | NISCAIR-CSIR, India |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Trypanosomosis or surra is caused by the haemoflagellate parasite, Trypanosoma evansi and is an important disease of animals, including domestic and wild herbivores and carnivores, in tropical countries. The invariant surface glycoproteins (ISGs) are blood stream stage specific and are uniformly distributed over the entire surface of the trypanosomes. In the present study, the extracellular domain (ED) region of ISG-75 from T. evansi, consisting of 1320 nt, encoding a polypeptide of 440 amino acids, has been heterologously expressed in Escherichia coli. Further, the immunoreactivity of recombinant ISG-75 (rISG-75) was characterized in immunoblot and ELISA using T. evansi hyper immune sera raised in experimental animals. The protein was found immunoreactive when compared with a panel of antigens (VSG RoTat 1.2 and whole cell lysate) using bovine serum samples from field. The diagnostic potential of rISG-75 was evaluated in ELISA with large number of bovine field serum samples. The optimum sensitivity and specificity were 98.47 and 99.1, respectively. The present finding showed that the expressed protein has potential use in the serodiagnosis of trypanosomosis. |
| Description: | Not Available |
| ISSN: | 0019-5189 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Experimental Biology |
| NAAS Rating: | 6.78 |
| Volume No.: | 53(1) |
| Page Number: | 7-15 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://nopr.niscair.res.in/handle/123456789/30275 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/8992 |
| Appears in Collections: | AS-NIVEDI-Publication |
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