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http://krishi.icar.gov.in/jspui/handle/123456789/9334| Title: | Field evaluation of Lateral Flow Assay in investigation brucellosis outbreak in buffalo farm of North India |
| Other Titles: | Not Available |
| Authors: | Shome R Filia G Padmashree BS Krithiga N Sahay S Triveni K Shome BR Mahajan V Singh A Rahman H |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2015-04-01 |
| Project Code: | Not Available |
| Keywords: | Brucellosis outbreak Competitive enzyme-linked immunoassay Indirect enzyme-linked immunoassay Lateral flow assay Rose bengal plate test |
| Publisher: | Veterinary World |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Aim: The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm. Materials and Methods: A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and competitive ELISA (cELISA). The performances of LFA and other serological tests were evaluated using OIE complaint cELISA as the gold standard. Results: Serological tests revealed 50% of the animals were seropositive for Brucella antibodies and correlated with clinical history of abortions, infertility, and productive failures. The newly developed assay showed 87.1% and 92.6% sensitivity and specificity, which was even higher than the specificity of RBPT. Conclusions: The investigation proved the potential usefulness of LFA for field diagnosis of brucellosis in the regions where laboratory facilities are limited. |
| Description: | Not Available |
| ISSN: | 2231-0916 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Veterinary World |
| NAAS Rating: | Not Available |
| Volume No.: | 8(4) |
| Page Number: | 492-496 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://www.veterinaryworld.org/Vol.8/April-2015/11.pdf |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/9334 |
| Appears in Collections: | AS-NIVEDI-Publication |
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