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http://krishi.icar.gov.in/jspui/handle/123456789/25945
Title: | Molecular characterization and evaluation of antibiotic susceptibility and biofilm forming capacity of pathogenic Escherichia coli isolated from seafood |
Other Titles: | Not Available |
Authors: | Safeena, M.P. Sreesudha, N.V. Larlyn, K. Pankaj Kishore Blossom, K.L. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Kerala University of Fisheries and Ocean Studies, Panangad, Kochi, Kerala, India ICAR::Central Institute of Fisheries Technology |
Published/ Complete Date: | 2017-11-21 |
Project Code: | Not Available |
Keywords: | Not Available |
Publisher: | ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch |
Citation: | Safeena, M.P.,Sreesudha, N.V.,Larlyn, K.,Pankaj Kishore and Blossom, K.L. (2017) Molecular characterization and evaluation of antibiotic susceptibility and biofilm forming capacity of pathogenic Escherichia coli isolated from seafood. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 415. |
Series/Report no.: | Not Available; |
Abstract/Description: | The present study made an attempt to investigate the occurrence of pathogenic E. coli in seafood, its antimicrobial susceptibility patterns and the biofilm forming capacity. In the present study, 43 seafood samples collected from commercial markets of Cochin were screened for the presence of pathogenic E. coli. The 21 biochemically confirmed isolates were further confirmed by PCR using various gene specific primers. The PCR results revealed that out of 21 isolates screened for the presence EIEC, EPEC, ETEC and EHEC strains of E. coli, only one isolate from fish sample (ECF5) showed positive results for rfbE gene of EHEC strain with a specific band of 213 bp on agarose gel. Antibiotic susceptibility pattern of the isolates revealed that 90 per cent of the isolates were found to be multidrug resistance to more than 5 antibiotics, whereas all the isolates showed sensitivity to doxycycline, naladixic acid, and azithromycin. The MAR index of all the isolates was found between 0.2 and 0.5. Even though all the isolates exhibited complete resistance to trimethoprim with no MIC level, the MIC observed for ciprofloxacin and for polymyxin-B and gentamicin was 0.016mcg per ml and 128 mcg per ml, respectively. Out of the 15 moderate biofilm forming E. coli obtained, 2 of them exhibited maximum multidrug resistance to more than 7 antibiotics whereas remaining 13 isolates showed multidrug resistance to 4-5 antibiotics. Hence this study revealed the emergence of antibiotic resistance in biofilm forming E. coli in seafood from Cochin markets, which may pose future threat to develop control strategies in the fish processing industry and also pose significant treatment challenge to physicians. |
Description: | Not Available |
ISBN: | 978-81-933623-1-0 |
Type(s) of content: | Other |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 415 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/25945 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
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415_Molecular characterization.pdf | 222.33 kB | Adobe PDF | View/Open |
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