Influence of thiamine and pyridoxine loaded vanillic acid grafted chitosan microparticles (TPVGC) on anti-fatigue activity in male Wistar strain albino rats through swimming induced stress

Abstract

The present investigation was conducted to study the influence of thiamine and pyridoxine loaded vanillic acid grafted chitosan microparticles (TPVGC) on antifatigue activity in male Wistar strain albino rats through swimming induced stress. Animals were distributed into four experimental groups which were fed varied levels of TPVGC for 45 days viz. Control (C); T1 ; T2 ; T3 . The animals were exposed to swimming exercise during the experimental period of 45 days. At the end of the feeding trial, the stress mitigation role of TPVGC was assessed. Average swimming time and anti-fatigue activity during the experimental period revealed that the animals fed with graded level of TPVGC had more stamina and swimed actively. Whereas, animals in the control group fed without TPVGC were more lethargic after 30 min of swimming stress. Metabolic responses were assessed in terms of lactate dehydrogenase, malate dehydrogenase, catalase, superoxide dismutase and acetylcholine esterase. LDH, MDH, catalase and SOD activities showed significant differences between the control and treatment group. The treatment group fed with graded level of TPVGC reduced enzyme activities. However, a reverse trend was observed for acetylcholine esterase. It was inferred that dietary supplementation of thiamine and pyridoxine loaded vanillic acid-grafted chitosan has stress mitigation role and enhanced stamina during swimming exercise.