Comparison of Collagen Extracted from Skin of Doublespotted Queenfish and Malabar Grouper

Abstract

Acid soluble collagen (ASC) and pepsin digestible collagen (PDC) from the skin of double-spotted queenfish (Scomberoides lysan) and Malabar grouper (Epinephelus malabaricus), were isolated and characterized. On wet weight basis, the yields of ASC and PDC from queen fish and grouper were 7.82, 3.92, 12.5 and 6.49% respectively. Amino acid analysis revealed that they contained glycine as a major amino acid with high contents of alanine, proline and hydroxyproline. Based on sodium dodecylsulfate polyacrylamide gel electrophoretic patterns and subunit compositions, all were identified to be type 1 collagens when compared with calf skin type 1 collagen. α1, α2 and β chains were the major components of the presently isolated collagens. While comparing these two species, queen fish skin had good yield of collagen which could be served as an alternative source of collagen for different applications.