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http://krishi.icar.gov.in/jspui/handle/123456789/41639
Title: | Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments |
Other Titles: | Not Available |
Authors: | Dimpal Thakuria Neetu Shahi Atul Kumar Singh Victoria Chanu Khangembam Arvind Kumar Singh Satish Kumar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Directorate of Cold Water Fisheries Research |
Published/ Complete Date: | 2017-01-01 |
Project Code: | Not Available |
Keywords: | NA |
Publisher: | PLOS / Public Library of Science |
Citation: | Thakuria, D., Shahi, N., Singh, A.K., Chanu, K.V., Singh, A.K. and Kumar, S. (2017). Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments. PLoS ONE, 12(10): e0185892. https://doi.org/10.1371/journal.pone.0185892. |
Series/Report no.: | Not Available; |
Abstract/Description: | Kisspeptin 1 is a neuropeptide hormone of the RFamide family, which act as an upstream regulator of brain-pituitary-gonad (BPG) axis in most vertebrates including teleosts. In the present study, a 16 amino acid long putative mature bioactive peptide (kiss 1) from preprokisspeptin 1 of golden mahseer, Tor putitora (Hamilton, 1822), was synthesized and characterized using an integrated (experimental and in silico) approach. The far-UV circular dichroism (CD) spectrum of this peptide was evaluated both in aqueous and membrane mimicking solvents (TFE, HFIP and Dioxane). The results indicate that kiss 1 peptide adopted helical, turn and β conformations in membrane like environments. The near-UV CD spectroscopy was also carried out to examine the tertiary packing around aromatic residues of kiss 1 peptide and the peptide-membrane complex. The kiss 1 peptide exhibited little signal in water, but a prominent negative band was observed at around 275 nm when membrane mimetic solution was added. The observed ordered conformations of kiss 1 peptide in the different solvents indicated its potential biological activity which could enhance the secretion of gonadotropin-releasing hormone (GnRH) at BPG axis. The conformational information generated from the present study reinforces the application prospects of bioactive synthetic peptide analogs of kisspeptin 1 in improving the reproductive performances of important cultivable fish species. |
Description: | Not Available |
ISSN: | 1932-6203 |
Type(s) of content: | Research Paper |
Sponsors: | Indian Council of Agricultural Research (ICAR) |
Language: | English |
Name of Journal: | PLOS One |
NAAS Rating: | 8.74 |
Volume No.: | 12(10) |
Page Number: | e0185892 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1371/journal.pone.0185892 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/41639 |
Appears in Collections: | FS-DCWFR-Publication |
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