Destructive dose determination of electron beam irradiation for pathogenic bacteria in water medium by 96 well plate assay

Abstract

Aim:Determination of suitable dosage for eradication of different pathogenic bacteria in water will be useful for further detailed research in this regard. Methodology: In the current investigation, known concentration of different actively growing bacterial culture were inoculated in 96- well-plate and exposed to 1, 2 and 3 kGy Electron Beam (EB) irradiation to understand the suitable destructive dosage. Results: 1 kGy EB irradiation completely destroyed more than 2 × 107 number of E. coli, approximately 2 × 106 number of MRSA, 5 × 105 number of Salmonella, V. cholerae and S. aureus and 1 × 104 number of P. aeruginosa, respectively. Based on the study, the hierarchy of susceptible bacteria was as follows: E. coli < MRSA < V. cholerae < S. aureus < Salmonella < P. aeruginosa < B. cereus < L. monocytogenes.Interpretation: The dose required for elimination of pathogens in 96 well plate was always lesser than that required for pathogens in the meat. 96-well-plate method is a simple and rapid method to assess different bacteria with different concentration in a single time and, hence, is useful and rapid technique to to determine the destructive irradiation dose for various bacteria and chemical pollutants in the substrate.