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http://krishi.icar.gov.in/jspui/handle/123456789/52574
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wazir Singh Lakra | en_US |
dc.contributor.author | M Singh | en_US |
dc.contributor.author | Mukunda Goswami | en_US |
dc.contributor.author | A Gopalakrishnan | en_US |
dc.contributor.author | K K Lal | en_US |
dc.contributor.author | V Mohindra | en_US |
dc.contributor.author | U K Sarkar | en_US |
dc.contributor.author | P P Punia | en_US |
dc.contributor.author | K V Singh | en_US |
dc.contributor.author | J P Bhatt | en_US |
dc.contributor.author | S Ayyappan | en_US |
dc.date.accessioned | 2021-08-03T08:31:37Z | - |
dc.date.available | 2021-08-03T08:31:37Z | - |
dc.date.issued | 2015-12-24 | - |
dc.identifier.citation | Lakra, W.S., Singh, M., Goswami, M., Gopalakrishnan, A., Lal, K.K., Mohindra, V., Sarkar, U.K., Punia, P.P., Singh, K.V., Bhatt, J.P. and Ayyappan, S. (2016) DNA barcoding Indian freshwater fishes, Mitochondrial DNA Part A, 27:6, 4510-4517, DOI: 10.3109/19401736.2015.1101540 | en_US |
dc.identifier.issn | 2470-1394 | - |
dc.identifier.issn | 2470-1408 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/52574 | - |
dc.description | Not Available | en_US |
dc.description.abstract | DNA barcoding is a promising technique for species identification using a short mitochondrial DNA sequence of cytochrome c oxidase I (COI) gene. In the present study, DNA barcodes were generated from 72 species of freshwater fish covering the Orders Cypriniformes, Siluriformes, Perciformes, Synbranchiformes, and Osteoglossiformes representing 50 genera and 19 families. All the samples were collected from diverse sites except the species endemic to a particular location. Species were represented by multiple specimens in the great majority of the barcoded species. A total of 284 COI sequences were generated. After amplification and sequencing of 700 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.40%, 9.60%, 13.10%, and 17.16%, respectively. DNA barcode discriminated congeneric species without any confusion. The study strongly validated the efficiency of COI as an ideal marker for DNA barcoding of Indian freshwater fishes. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Taylor & Francis | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | DNA barcode | en_US |
dc.subject | COI | en_US |
dc.subject | Genetic divergence | en_US |
dc.subject | Phylogeny | en_US |
dc.title | DNA barcoding Indian freshwater fishes | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Mitochondrial DNA Part A - DNA Mapping, Sequencing & Analysis | en_US |
dc.publication.volumeno | 27(6) | en_US |
dc.publication.pagenumber | 4510-4517 | en_US |
dc.publication.divisionUnit | Molecular Biology & Biotechnology | en_US |
dc.publication.sourceUrl | https://doi.org/10.3109/19401736.2015.1101540 | en_US |
dc.publication.sourceUrl | https://www.tandfonline.com/doi/abs/10.3109/19401736.2015.1101540?journalCode=imdn21 | en_US |
dc.publication.authorAffiliation | ICAR-Central Institute of Fisheries Education, Versova, Mumbai | en_US |
dc.publication.authorAffiliation | Department of Zoology and Biotechnology, Hemwati Nandan Bahuguna Garhwal University, Srinagar, Uttarakhand – 246174, India | en_US |
dc.publication.authorAffiliation | Indian Council of Agricultural Research, New Delhi, India | en_US |
dc.publication.authorAffiliation | ICAR-National Bureau of Fish Genetic Resources, Lucknow | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. | en_US |
dc.publication.naasrating | 7.07 | en_US |
dc.publication.impactfactor | 1.514 | en_US |
Appears in Collections: | FS-NBFGR-Publication |
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