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http://krishi.icar.gov.in/jspui/handle/123456789/52705| Title: | Detection of Listeria monocytogenes from freshwater fish,prawn and chicken meat by direct nested PCR |
| Other Titles: | Not Available |
| Authors: | Sanjoy Das Singh,V.P. Keduzol Ltu Kathiresan,S. Bhaskar Sharma Bhilegaonkar, K.N. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute ICAR::Central Institute of Fisheries Technology |
| Published/ Complete Date: | 2011 |
| Project Code: | Not Available |
| Keywords: | Not Available |
| Publisher: | Asian Fisheries Society |
| Citation: | Sanjoy Das, Singh,V.P., Keduzol Ltu, Kathiresan,S., Bhaskar Sharma, and Bhilegaonkar, K.N. (2011) Detection of Listeria monocytogenes from freshwater fish,prawn and chicken meat by direct nested PCR, Asian Fish. Sci., 24:432-442. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Three different methods of processing samples from freshwater fish, prawn and chicken meat were compared with nested PCR for the detection of Listeria monocytogenes. Nested PCR failed to detect the organism from freshwater fish, prawn and chicken meat when processing was done by boiling lysis method, but it could detect the organisms from freshwater fish, prawn and chicken meat up to the spiking level of 104, 103 and 105 cfu . g (raised to minus one) , respectively while processing of sample was done by phenol extraction without enrichment. However, using phenol extraction after enrichment, the sensitivity was found higher than without enrichment and it was possible to detect up to the spiking level of 10 (raised to two) , 10 and 10 (raised to three) cfu . g (raised to minus one) in case of fish, prawn and chicken meat, respectively. |
| Description: | Not Available |
| ISSN: | 0116-6514 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Asian Fisheries Science |
| Journal Type: | International Journal |
| Impact Factor: | 0 |
| Volume No.: | 24 |
| Page Number: | 432-442 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/52705 |
| Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Detection of Listeria monocytogenes from freshwater fish.pdf | 343.35 kB | Adobe PDF | View/Open |
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