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http://krishi.icar.gov.in/jspui/handle/123456789/78042
Title: | Effect of Electron Beam Irradiation in Combination with Other Treatments on Shrimp Allergen, Tropomyosin |
Other Titles: | Not Available |
Authors: | Laly, S.J. Jeyakumari, A. Kuppa Sivasankara Sarma Kaushlesh Pansingh Rawat Shaik Abdul Khader Sankar T. V. Panda, S. K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology Electron Beam Processing Section, IRAD, BARC, BRIT/BARC Complex, Navi Mumbai, Maharashtra, 400703, India |
Published/ Complete Date: | 2022-08-10 |
Project Code: | Not Available |
Keywords: | Electron beam irradiation IgE activity Immunoblotting Shrimp tropomyosin |
Publisher: | Springer |
Citation: | Laly, S.J., Jeyakumari, A., Kuppa Sivasankara Sarma, Kaushlesh Pansingh Rawat, Shaik Abdul Khader, Sankar T. V. and Panda, S. K. (2022) Effect of Electron Beam Irradiation in Combination with Other Treatments on Shrimp Allergen, Tropomyosin. In: Lama, T., Burman, D., Mandal, U.K., Sarangi, S.K., Sen, H. (eds) Transforming Coastal Zone for Sustainable Food and Income Security. Springer, Cham. https://doi.org/10.1007/978-3-030-95618-9_44. 577-586. |
Series/Report no.: | Not Available; |
Abstract/Description: | A hurdle technology-based approach for the reduction in immunogenicity of shrimp major allergen, tropomyosin was investigated by combining electron beam irradiation at 5 kGy and boiling, autoclaving, trypsin and chymotrypsin treatments along with peeled raw shrimp as control. Shrimp extracts were prepared from the irradiated sample and evaluated SDS PAGE profile, IgE binding ability and immunogenicity of tropomyosin using pooled sera of shrimp sensitive individuals. Electron beam irradiation resulted in significant (p < 0.05) decrease in the IgE activity in the case of all treatments. The SDS PAGE analysis showed the presence of tropomyosin in all the treatments except autoclaving in combination with or without electron beam irradiation. A pronounced reduction in allergenicity was observed in the treatment of autoclaving in combination with electron beam irradiation. The combined application of autoclaving and electron beam irradiation can be utilized for the preparation of hypoallergic shrimp. |
Description: | Not Available |
ISBN: | 978-3-030-95618-9 |
Type(s) of content: | Book chapter |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 577-586. |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1007/978-3-030-95618-9_44 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/78042 |
Appears in Collections: | FS-CIFT-Publication |
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