In vivo assessment of wound closure and biocompatibility of nanochitosan-polyvinylalcohol-glycerol films in experimental animal model

Abstract

The need for innovative wound dressings has arisen due to injuries from second intention healing and their management is associated with high costs. Modern dressings not only protect wounds but also promote proliferation of cells and tissue regeneration. Patient compliance is crucial, ensuring that the dressing removal does not harm the newly regenerated tissue. In this study, nano-chitosan polyvinyl alcohol- glycerol (NCH-PVA-GLY) transparent wound dressing was developed that expedites tissue repair and can be removed through an external stimulus without disrupting the regenerated tissue. This dressing efficiently absorbs excess wound exudates while maintaining proper hydration. Evaluation in male Wistar rats with wounds revealed that the dressing, when applied for 16 days, resulted in minimal inflammation and denser connective tissue compared to wounds devoid of dressings. The dressing exhibited enhanced re-epithelization and angiogenesis, facilitating effective wound closure. The exposure of the resultant dressing, to the incised wound facilitated complete wound closure in 16 days, suggesting the effectiveness of the dressings to cure superficial wound.