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http://krishi.icar.gov.in/jspui/handle/123456789/83876
Title: | Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India |
Other Titles: | Not Available |
Authors: | Vineeth Rajan Ardhra Vijayan Ambili P. M. Justus Lancy Sivaraman, G. K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology Government Medical College, Ernakulam, India |
Published/ Complete Date: | 2023-07-04 |
Project Code: | Not Available |
Keywords: | Acinetobacter baumannii Biofilm formation Carbapenem resistance Disinfectant resistance Pulsed-field gel electrophoresis |
Publisher: | Microbiology Society |
Citation: | Vineeth Rajan, Ardhra Vijayan, Ambili P. M., Justus Lancy and Sivaraman, G. K. (2023) Genotyping of Acinetobacter baumannii isolates from a tertiary care hospital in Cochin, South India. Access Microbiology, 5(11): 1-20. |
Series/Report no.: | Not Available; |
Abstract/Description: | Acinetobacter baumannii poses a significant challenge in healthcare settings across the globe, with isolates exhibiting carbap enem resistance at unprecedented rates. Here, we characterized a collection of A. baumannii isolates (n=64) recovered during the period September 2020 – November 2021 at a teaching hospital in Cochin, South India. The species identity of the isolates was confirmed with blaOXA-51-like PCR. The major carbapenemase determinants identified were blaOXA-23-like (45, 70.3%) and blaNDM-1 (31, 48.4%); co-occurrence of these genes was also observed in 27 (42.2%) isolates. Other resistance genes identified included blaPER (34, 53.1%), aac(6')-Ib-cr (42, 65.6%), qnrS (25, 39.1%), sul1 (32, 50%), sul2 (33, 51.6%), strA/strB (36, 56.3%), aphA1-Iab (35, 54.7%) and tetB (32, 50%). Mapping PCR revealed the insertion element, ISAbaI upstream of blaOXA-23-like in all isolates possessing this gene. Concerning disinfectant resistance, all isolates carried the quaternary ammonium compound (QAC) resistance gene, qacEΔ1. Minimal inhibitory concentration (MIC) of benzalkonium chloride was high among the isolates and ranged from 8 to 128µgml−1. However, low MICs were observed for chlorhexidine and triclosan, with the majority (54, 80.6%) of isolates showing an MIC of 2µgml−1 for chlorhexidine and all isolates exhibiting MICs of ≤0.125µgml−1 for triclosan. Further, all isolates were strong biofilm-producers, as assessed by the crystal violet-based microtitre plate assay. The ApaI-pulsed-field gel electropho resis (PFGE) revealed the multi-clonal nature of the isolates, with 16 clusters and 16 unique pulsotypes identified at a cut-off of 80%. In short, this study provides useful data on the molecular features of A. baumannii from this region, which could be helpful to assess the local epidemiology of this pathogen and also to devise infection control strategies. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Access Microbiology |
Journal Type: | Not Available |
NAAS Rating: | 0 |
Impact Factor: | 0 |
Volume No.: | 5(11) |
Page Number: | 1-20 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1099/acmi.0.000662.v1. |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/83876 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
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Genotyping of Acinetobacter baumannii isolates from a tertiary.pdf | 4.35 MB | Adobe PDF | View/Open |
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