Record Details

Molecular detection and in silico characterization of cold shock protein coding gene (cspA) from cold adaptive Pseudomonas koreensis

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular detection and in silico characterization of cold shock protein coding gene (cspA) from cold adaptive Pseudomonas koreensis
Not Available
 
Creator Srikant Awasthi
Anjney Sharma
Pragya Saxena
Jagriti Yadav
K. Pandiyan
M. Kumar
Arjun Singh
Hillol Chakdar
Arpan Bhowmik
Prem L. Kashyap
Alok K. Srivastava
Anil K. Saxena
 
Subject Cold shock protein
Pseudomonas koreensis
Psychrophiles
Gene expression
Molecular modeling
 
Description Not Available
Cold shock proteins (CSPs) are greatly conserved family of structurally related DNA binding proteins which are produced
during temperature drift. A 213 bp long cspA gene was cloned and sequenced from Pseudomonas koreensis P2 in the
present study. The expression analysis of the cspA showed 2.5 folds increase in the mRNA level at 15 C while the
expression was almost on par at 30 C and 5 C indicating its role in moderately low temperature. In silico analyses of the
gene showed that the gene codes for 7.69 kDa protein which was phylogenetically very similar to CspA present in
Pseudomonads. Amino acid composition of the CspA from P. koreensis was different from that of mesophilic Pseudomonas
and tiny/small amino varied significantly between CspA of cold adaptive and mesophilic species. The CspA from
P. koreensis P2 contained RNP motifs involved in binding of DNA and RNA. Phylogenetic analyses revealed that the
CspA protein of P. koreensis P2 was more close to CspA of distant subgroups of Pseudomonas like P. fluorescens and P.
putida subgroup indicating a possible intra-specific gene transfer.
The authors gratefully acknowledge the financial assistance under network project ‘Application of Microorganisms Agriculture and Allied Sectors (AMAAS)’ and ‘‘CRP Genomics’’ from Indian Council of Agricultural Research (ICAR), India. in
 
Date 2020-05-25T01:31:10Z
2020-05-25T01:31:10Z
2019-03-27
 
Type Research Paper
 
Identifier Awasthi, S., Sharma, A., Saxena, P., Yadav, J., Pandiyan, K., Kumar, M., Singh, A., Chakdar, H., Bhowmik, A., Kashyap, P.L., Srivastava, A.K. and Saxena, A.K. Molecular detection and in silico characterization of cold shock protein coding gene (cspA) from cold adaptive Pseudomonas koreensis. Journal of Plant Biochemistry and Biotechnology. DOI 10.1007/s13562-019-00500-8
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/36248
 
Language English
 
Relation Not Available;
 
Publisher Journal of Plant Biochemistry and Biotechnology