ICAR Research Data Repository for Knowledge Management
Expression of Rhizobium meliloti symbiotic promoters in Azorhizobium caulinodans.
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Expression of Rhizobium meliloti symbiotic promoters in Azorhizobium caulinodans.
Not Available |
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| Creator |
Das Avijit, Khanuja SPS and Lodha ML
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| Subject |
oxygen sensitive, Azorhizobium caulinodans, azide tolerance, expression, symbiotic promoter,Rhizobium meliloti, nitrogen fixation
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| Description |
Not Available
Plasmids containing Rhizobium meliloti symbiotic promoters P1 (promoter of nifHDK) and P2 (promoter of fixABCX) when mobilized into the cells of Azorhizobium caulinodans strain IRBG 46 showed strong expression of these promoters under free-living microaerobic as well as symbiotic conditions. Under free-living conditions microaerobiosis (3% or less O2) was found to be sufficient to activate these promoters; expression being higher at 1% than at 3% O2 concentration. Under symbiotic conditions the expression was much more stronger-with bacteroids in stem nodules showing higher expression than those in root nodules. Under both the conditions expression of the promoters in the native R. meliloti strain Rm102F34 was lower than that in the A. caulinodans strain IRBG 46. The results suggest a functional homology of these promoters in the heterologous background of A. caulinodans. Not Available |
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| Date |
2021-04-30T05:57:21Z
2021-04-30T05:57:21Z 1997-01-01 |
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| Type |
Article
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/46879 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Society for Plant Biochemistry and Biotechnology
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