Multi track staining apparatus with high resolution and time-lapsed acquisition for polyacrylamide gels.
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Title |
Multi track staining apparatus with high resolution and time-lapsed acquisition for polyacrylamide gels.
Not Available |
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Creator |
Maurye P.
Basu A. Biswas J.K. |
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Subject |
polyacrylamide gel
proteins nucleic acids silver staining |
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Description |
Not Available
Polyacrylamide gel electrophoresis (PAGE) is commonly used for the separation, identification, and purification of mixtures of proteins and nucleic acids. A suitable staining method for the polyacrylamide gels is imperative to determine low concentrations of resolved macromolecules. Several staining methods have been adopted; ethidium bromide, silver, and coomassie staining are the most frequently employed. Silver staining determines macromolecules at nanomolar concentrations but involves many steps and thus is tedious for multiple gels in parallel. In this article, a simple multitrack laboratory-constructed silver staining apparatus is characterized that may enhance the sensitivity of the electrophoresis stains and the homogeneity of detection. In addition, the low cost and simplicity of this system provide wide applicability for gel electrophoresis. Not Available |
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Date |
2018-12-31T06:00:36Z
2018-12-31T06:00:36Z 2015-03-18 |
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Type |
Research Paper
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Identifier |
Maurye P., Basu A. and Biswas J.K. 2015. Multi track staining apparatus with high resolution and time-lapsed acquisition for polyacrylamide gels. Instrumentation Science & Technology. DOI: 10.1080/10739149.2015.1027919.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/16337 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Taylor & Francis
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