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Identification of Heat-Related ESTs in Moth Bean Through Suppression Subtraction Hybridization

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Title Identification of Heat-Related ESTs in Moth Bean Through Suppression Subtraction Hybridization
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Creator Karun Gurjar
Sakshi Rampuria
Uma Joshi
Paramita Palit
K. V. Bhatt
T. Mohapatra
Ramavtar Sharma
 
Subject Moth bean
SSH cDNA library
Sequence analysis
HSPs
Semi-qRT-PCR
assay
 
Description Not Available
Abstract Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant genotype RMO-40 to identify genes expressing under delayed response to elevated temperature. Heat induction was carried out by exposing 14-day-old seedlings to elevated temperature of 42 °C for 30 min. A total of 125 unigenes (33 contigs and 92 singletons) were derived by cluster assembly and sequence
alignment of 200 ESTs; out of 125 unigenes, 21 (16 %) were found to be novel to moth bean. Gene ontology functional classification terms were retrieved for 98 (78.4 %) unigenes of
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Date 2020-12-23T11:20:45Z
2020-12-23T11:20:45Z
2014-06-01
 
Type Research Paper
 
Identifier 66. Karun Gurjar & Sakshi Rampuria & Uma Joshi & Paramita Palit & K. V. Bhatt & T. Mohapatra & Ramavtar Sharma. 2014. Identification of Heat-Related ESTs in Moth Bean through Suppression Subtraction Hybridization. Appl Biochem Biotechnol 173 (8), 2116-2128. DOI 10.1007/s12010-014-1011-2
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http://krishi.icar.gov.in/jspui/handle/123456789/43606
 
Language English
 
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Publisher Not Available