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CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops

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Title CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops
 
Creator Karkute S.G., Singh A.K., Gupta O.P., Singh P.M. and Singh B.
 
Subject CRISPR/Cas9, genome editing, vegetables, fruits, horticultural crops
 
Description Not Available
Horticultural crops are an important part of agriculture for food as well as nutritional security. However, several pests and diseases along with adverse abiotic environmental factors pose a severe threat to these crops by affecting their quality and productivity. This warrants the effective and accelerated breeding programs by utilizing innovative biotechnological tools that can tackle aforementioned issues. The recent technique of genome editing by Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/Cas9) has greatly advanced the breeding for crop improvement due to its simplicity and high efficiency over other nucleases such as Zinc Finger Nucleases and Transcription Activator Like Effector Nucleases. CRISPR/Cas9 tool contains a non-specific Cas9 nuclease and a single guide RNA that directs Cas9 to the specific genomic location creating double-strand breaks and subsequent repair process creates insertion or deletion mutations. This is currently the widely adopted tool for reverse genetics, and crop improvement in large number of agricultural crops. The use of CRISPR/Cas9 in horticultural crops is limited to few crops due to lack of availability of regeneration protocols and sufficient sequence information in many horticultural crops. In this review, the present status of applicability of CRISPR/Cas9 in horticultural crops was discussed along with the challenges and future potential for possible improvement of these crops for their yield, quality, and resistance to biotic and abiotic stress
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Date 2019-07-15T06:36:39Z
2019-07-15T06:36:39Z
2017-09-22
 
Type Article
 
Identifier Karkute SG, Singh AK, Gupta OP, Singh PM and Singh B (2017) CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops. Front. Plant Sci. 8:1635. doi: 10.3389/fpls.2017.01635
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http://krishi.icar.gov.in/jspui/handle/123456789/21477
 
Language English
 
Relation Not Available;
 
Publisher Frontiers in Plant Science