ICAR Research Data Repository for Knowledge Management
Detection of viruses infecting black pepper by SYBR Green-based real-time PCR assay
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View Archive Info| Field | Value | |
| Title |
Detection of viruses infecting black pepper by SYBR Green-based real-time PCR assay
Not Available |
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| Creator |
Bhat, A.I. and Siljo, A. 2014.
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| Subject |
Cucumber mosaic virus
Piper yellow mottle virus Real-time PCR Real-time RT-PCR Detection Sensitivity |
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| Description |
Not Available
Virus infections are a major constraint to cultivation of black pepper (Piper nigrum L.), a perennial vegetatively propagated spice crop. Real-time PCR and real-time RTPCR were developed to detect Piper yellow mottle virus (PYMoV) and Cucumber mosaic virus (CMV), respectively, in black pepper plants. These detection assays were up to 1000 times more sensitive than the conventional PCR/RTPCR assays. Field samples from different pepper-growing regions in India were used to validate the two detection assays, which identified more infected plants than conventional PCR. Our results indicate that real-time PCR should be the method of choice for screening mother plants for PYMoV and CMV. Not Available |
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| Date |
2019-11-30T11:22:43Z
2019-11-30T11:22:43Z 2014 |
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| Type |
Research Paper
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| Identifier |
Bhat, A.I. and Siljo, A. 2014. Detection of viruses infecting black pepper by SYBR Green-based real-time PCR assay. Journal of Plant Pathology, 96: 105-109.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/26058 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Italian Phytopathological Society
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