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Artificial microRNA-derived resistance to Cassava brown streak disease

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Title Artificial microRNA-derived resistance to Cassava brown streak disease
Not Available
 
Creator Henry Wagaba
Basavaprabhu L. Patil
Settumba Mukasa
Titus Alicai
Claude M. Fauquet
Nigel J. Taylor
 
Subject Artificial microRNA
Cassava brown streak disease
 
Description Not Available
Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3'-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20-60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava.
Not Available
 
Date 2019-03-20T03:44:13Z
2019-03-20T03:44:13Z
2016-06-01
 
Type Research Paper
 
Identifier H. Wagaba, B. L. Patil, S. Mukasa, T. Alicai, C. M. Fauquet and N. J. Taylor. (2016) Artificial microRNA-derived resistance to Cassava brown streak disease. Journal of Virological Methods. 231, 38-43.
http://dx.doi.org/10.1016/j.jviromet.2016.02.004
http://krishi.icar.gov.in/jspui/handle/123456789/17518
 
Language English
 
Relation Not Available;
 
Publisher Elsevier