Record Details

Molecular cloning and in silico analysis of DREB-like gene in watermelon

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular cloning and in silico analysis of DREB-like gene in watermelon
Chet Ram , Manjusha Verma, Bhupendra Singh Panwar and Lalit Arya
 
Creator Not Available
 
Subject Drought tolerance
DREB
in silico analysis
watermelon
 
Description Not Available
The objective of this study was to identify and characterize sequences from watermelon related to drought
tolerant genes. In order to achieve this, publicly available Cucumis melo REB gene (Cme-DREB1) sequence was
retrieved from NCBI database and gene-specific primers were synthesized. A fragment of 274 bp corresponding
to DREB-like gene was amplified from genomic DNA of watermelon. PCR product was cloned in E. coli vector
(pDrive) and validated through sequencing. The BLAST search showed 99 and 97% homology to CmeDREB1
gene and C. sativus DREB3-like (CsDREB3-like) gene, respectively. Furthermore, it showed 87 to 93% homology
with rest of DREB genes available in the public database. Full length watermelon DREB-like gene (C. lanatus
DREB-like, ClaDREB-like) was identified from cucurbit genomic database and investigated through in silico
analysis. The intronless structural organization of ClaDREB-like gene was found with a conserved AP2/ERF
domain.
Not Available
 
Date 2019-04-06T11:35:35Z
2019-04-06T11:35:35Z
2015-09-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/18053
 
Language English
 
Relation Not Available;
 
Publisher Indian Society of Horticulture New Delhi