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De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines

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Title De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines
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De Novo Transcriptomes Assembly of Bitter Gourd
 
Creator Not AvailaAnjali Shukla, V. K. Singh, D. R. Bharadwaj, Rajesh Kumar, Ashutosh Rai, A. K. Rai, Raja Mugasimangalam, Sriram Parameswaran, Major Singh, P. S. Naikble
 
Subject Bitter Gourd, Transcriptome, Gynoecious and Monoecious
 
Description Not Available
Bitter gourd (Momordica charantia L.) is a nutritious vegetable crop of Asian origin, used as
a medicinal herb in Indian and Chinese traditional medicine. Molecular breeding in bitter
gourd is in its infancy, due to limited molecular resources, particularly on functional markers
for traits such as gynoecy. We performed de novo transcriptome sequencing of bitter gourd
using Illumina next-generation sequencer, from root, flower buds, stem and leaf samples of
gynoecious line (Gy323) and a monoecious line (DRAR1). A total of 65,540 transcripts for
Gy323 and 61,490 for DRAR1 were obtained. Comparisons revealed SNP and SSR variations
between these lines and, identification of gene classes. Based on available transcripts
we identified 80 WRKY transcription factors, several reported in responses to biotic and abiotic
stresses; 56 ARF genes which play a pivotal role in auxin-regulated gene expression
and development. The data presented will be useful in both functions studies and breeding
programs in bitter gourd.
ICAR
 
Date 2020-09-10T09:12:46Z
2020-09-10T09:12:46Z
2015-06-05
 
Type Research Paper
 
Identifier Shukla Anjali, Singh VK, Bharadwaj DR, Kumar Rajesh, Rai Ashutosh, Rai AK, Mugasimangalam Raja, Parameswaran Sriram, Singh Major, Naik PS (2015). De novo assembly of bitter gourd transcriptomes: gene expression and sequence variations in gynoecious and monoecious lines. PLoS ONE 10(6): e0128331. doi:10.1371/journal.pone.0128331.
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http://krishi.icar.gov.in/jspui/handle/123456789/41132
 
Language English
 
Relation Not Available;
 
Publisher PLOS ONE