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Isolation and expression analyses of KLUH gene in developing seeds and enhanced seed oil in KLUH overexpressing Brassica juncea transgenics

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Title Isolation and expression analyses of KLUH gene in developing seeds and enhanced seed oil in KLUH overexpressing Brassica juncea transgenics
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Creator Siddanna Savadi, Deepak Singh Bisht, Shripad Ramachandra Bhat
 
Subject fatty acid; seed coat; KLUH; gene overexpression
 
Description Not Available
Improving oil yield of Indian mustard (Brassica juncea) is exigent as it is a major oilseed crop of Indian subcontinent, which has severe shortage of vegetable oil production in the world. Some of the regulators of seed development have been shown to improve oil yield in Arabidopsis. Arabidopsis KLUH (AtKLUH), a maternal regulator of seed size, has been shown to control seed oil content. In this study, we identified three homologs of AtKLUH in B. juncea, BjKLUH1, BjKLUH2-1 and BjKLUH2-2. We observed that BjKLUH1 differentially expresses in developing seeds in B. juncea accessions with varying seed size and oil content. Further, analyses for seed oil content in B. juncea transgenics carrying AtKLUH demonstrated an increase in seed oil up to 8.3% compared to wild-type plants. The results of this study suggest that KLUH may have a role in seed development and is a good candidate for engineering seed oil accumulation in B. juncea.
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Date 2019-12-03T05:27:59Z
2019-12-03T05:27:59Z
2017-09-26
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/26478
 
Language English
 
Relation Not Available;
 
Publisher De Gruyter