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Modifying DNA methylation pattern in papaya embryos to harness useful and stable variants.

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Title Modifying DNA methylation pattern in papaya embryos to harness useful and stable variants.
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Creator Kanupriya Chaturvedi
Manamohan, M
Leela Sahijram
 
Subject Carica papaya, MS-RAPD-PCR, zygotic embryo
 
Description Not Available
The purpose of this study was to investigate the effect of various DNA methylation modifying agents on
genome wide global DNA methylation status and to develop papaya cv. Surya plants with useful and stable
horticulturally important variations. DNA methylation-status modifying agents (Zebularine), 5-azacytidine, 5-aza
deoxycytidine (DNA hypomethylating agents), 3’-azido-deoxythymidine (DNA hypermethylating agent) were
used at two concentrations (0.3mM and 0.5mM) on papaya cv. Surya zygotic embryos for incubation periods
ranging from 0-120 hrs. The technique of MS-RAPD-PCR which resolves global DNA methylation pattern was
employed. A total of 87 primers were tested on the control samples, 55 primers showed PCR amplification, of
which 11 showed polymorphic bands. Six primers showing clear and reproducible bands were selected further
for analysing methylation-modifying-agent treated samples. This technique was found to be reliable and efficient for detecting changes in DNA methylation at both the scale and pattern in a genome wide standpoint.
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Date 2021-07-28T08:10:54Z
2021-07-28T08:10:54Z
2019-10-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/50632
 
Language English
 
Publisher HSI