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In Vitro Anti-Cervical Cancer Effect of Proteoglycans Isolated from Deep Sea Shark Cartilage by Inducing Apoptosis and Cell Cycle Arrest.

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Title In Vitro Anti-Cervical Cancer Effect of Proteoglycans Isolated from Deep Sea Shark Cartilage by Inducing Apoptosis and Cell Cycle Arrest.
Not Available
 
Creator Ajeeshkumar, K. K.
Vishnu, K.V.
Asha, K. K.
Remyakumari, K. R.
Reshma, J.
Suseela Mathew
 
Subject Proteoglycans
Shark cartilage
Apoptosis
Cell cycle arrest
 
Description Not Available
The present study was aimed at studying in vitro anti-cervical cancer effect of proteoglycans (PGs) isolated from deep sea shark. Anti-proliferative activity and apoptotic involvement were confirmed by following assays viz, the cell viability assay by MTT assay, morphological analysis and colony formation assay. The sample showed a significant cytotoxic activity for HeLa cells and results showed that the PGs at a concentration of 250μg/mL inhibit the colony formation capacity. Apoptosis induction was confirmed by the chromatin condensation assay by Hoechst 33342 nuclear staining. A number of apoptotic HeLa cells displayed a round and shrunken cell body and chromatin condensation inside the nucleus suggesting that PGs-induced apoptosis
caused cell death. Apoptosis of HeLa cells was further verified by annexin V-FITC (fluorescein isothiocyanate) and PI (propidium iodide) staining by flow cytometry. PGs were found to induce apoptosis and the ratio of apoptotic cells were 30.5%. Cell cycle arrest at G1/M and G2/M phase confirmed the cell death due to cell cycle arrest. Our study determined that proteoglycans effectively induced apoptosis and caused cell cycle arrestestablishing their antiproliferative effect against cervical cancer.
Not Available
 
Date 2020-06-02T06:44:39Z
2020-06-02T06:44:39Z
2017-12
 
Type Research Paper
 
Identifier Ajeeshkumar, K. K., Vishnu, K.V., Asha, K. K., Remyakumari, K. R., Reshma, J. And Suseela Mathew (2017) In Vitro Anti-Cervical Cancer Effect of Proteoglycans Isolated from Deep Sea Shark Cartilage by Inducing Apoptosis and Cell Cycle Arrest. International Journal of Development Research 7(12):17472-17478.
2230-9926
http://krishi.icar.gov.in/jspui/handle/123456789/36739
 
Language English
 
Relation Not Available;
 
Publisher International Journal of Development Research