ICAR Research Data Repository for Knowledge Management
Application of a loop-mediated isothermal amplification assay for rapid detection of cow components adulterated in buffalo milk/meat
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Application of a loop-mediated isothermal amplification assay for rapid detection of cow components adulterated in buffalo milk/meat
Not Available |
|
| Creator |
Not Available
Deb R, Sengar G S, Singh U, Kumar S, Raja T V, Alyethodi RR, Alex R, Das AK, Prakash B |
|
| Subject |
LAMP assay
Cow components Adulteration Buffalo milk/meat samples |
|
| Description |
Not Available
Loop-mediated isothermal amplification (LAMP) is a diagnostic method for amplification of DNA with rapid and minimal equipment requirement. In the present study, we applied the LAMP assay for rapid detection of cow components adulteration in buffalo milk/ meat samples. The test can be completed within around 1 h 40 min starting from DNA extraction and can be performed in water bath without requirement of thermocycler. The cow DNA in buffalo samples were identified in the developed LAMP assay by either visualizing with SYBR Green I/HNB dyes or observing the typical ladder pattern on gel electrophoresis. The test can detect up to 5 % level of cow milk/meat mixed in buffalo counterparts. Due to the simplicity and specificity, the developed LAMP test can be easily adapted in any laboratory for rapid detection of cow species identification in livestock by products. AICRP on Cattle,ICAR |
|
| Date |
2017-06-28T09:50:43Z
2017-06-28T09:50:43Z 2016-10-19 |
|
| Type |
Research Paper
|
|
| Identifier |
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/4497 |
|
| Language |
English
|
|
| Publisher |
Springer
|
|