ICAR Research Data Repository for Knowledge Management
Species identification of cattle and buffalo fat through PCR assay
KRISHI: Publication and Data Inventory Repository
View Archive Info| Field | Value | |
| Title |
Species identification of cattle and buffalo fat through PCR assay
Not Available |
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| Creator |
S. Vaithiyanathan and V. V. Kulkarni
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| Subject |
Cattle Buffalo Fat mt DNA PCR
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| Description |
Not Available
A method was standardized to isolate quality DNA from cattle and buffalo fat for species identification using QIAamp DNA stool mini kit. The quality of the DNA was sufficient enough to amplify universal primers viz., mt 12S rRNA and mt 16S rRNA, and species specific D loop primers for cattle and buffalo. The sensitivity of the PCR assay in the species specific D loop primer amplification was with a detection level of 0. 47 ng cattle DNA and 0.23 ng buffalo DNA in simplex and, 0. 47 ng cattle DNA and 0.12 ng buffalo DNA in duplex PCR. It is a potentially reliable method for DNA detection to authenticate animal fat. Not Available |
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| Date |
2018-09-27T08:43:57Z
2018-09-27T08:43:57Z 2016-12-31 |
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| Type |
Article
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/7308 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Springer Link
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