Detection of beef adulteration by mitochondrial D-loop based species-specific polymerase chain reaction.
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Detection of beef adulteration by mitochondrial D-loop based species-specific polymerase chain reaction.
Not Available |
|
Creator |
Nagappa K, Girish P.S, Kumar D and Singh S.P
|
|
Subject |
Beef, Adulteration, DNA, PCR, D-loop
|
|
Description |
Not Available
A species-specific polymerase chain reaction (PCR) was developed for the identification of beef that is cattle (Bos indicus) meat by targeting mitochondrial D-loop region. A pair of beef-specific primers was designed to amplify conserved region of D-loop and possibility of cross-amplification was ruled by taking into account other species comprising of mammals, birds, rodents and fish. The amplified PCR product of 381 bp was evident in raw (n = 20), cooked (60, 80 and 100°C), autoclaved (121°C) and micro-oven processed beef samples. Sensitivity of PCR assay was at 0.1% for detection of beef adulteration and the limit of detection (LOD) of beef DNA was 0.1 picograms (pg). Newly developed beef-specific PCR assay was rapid and sensitive and hence could be applied for the detection of adulteration of meat. Not Available |
|
Date |
2018-09-29T07:02:07Z
2018-09-29T07:02:07Z 2017-12-31 |
|
Type |
Article
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/7385 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Taylor & Francis Online
|
|