Field evaluation of Lateral Flow Assay in investigation brucellosis outbreak in buffalo farm of North India
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Title |
Field evaluation of Lateral Flow Assay in investigation brucellosis outbreak in buffalo farm of North India
Not Available |
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Creator |
Shome R
Filia G Padmashree BS Krithiga N Sahay S Triveni K Shome BR Mahajan V Singh A Rahman H |
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Subject |
Brucellosis outbreak
Competitive enzyme-linked immunoassay Indirect enzyme-linked immunoassay Lateral flow assay Rose bengal plate test |
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Description |
Not Available
Aim: The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm. Materials and Methods: A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and competitive ELISA (cELISA). The performances of LFA and other serological tests were evaluated using OIE complaint cELISA as the gold standard. Results: Serological tests revealed 50% of the animals were seropositive for Brucella antibodies and correlated with clinical history of abortions, infertility, and productive failures. The newly developed assay showed 87.1% and 92.6% sensitivity and specificity, which was even higher than the specificity of RBPT. Conclusions: The investigation proved the potential usefulness of LFA for field diagnosis of brucellosis in the regions where laboratory facilities are limited. Not Available |
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Date |
2018-11-05T09:08:26Z
2018-11-05T09:08:26Z 2015-04-01 |
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Type |
Research Paper
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Identifier |
Not Available
2231-0916 http://krishi.icar.gov.in/jspui/handle/123456789/9334 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Veterinary World
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