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Field evaluation of Lateral Flow Assay in investigation brucellosis outbreak in buffalo farm of North India

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Title Field evaluation of Lateral Flow Assay in investigation brucellosis outbreak in buffalo farm of North India
Not Available
 
Creator Shome R
Filia G
Padmashree BS
Krithiga N
Sahay S
Triveni K
Shome BR
Mahajan V
Singh A
Rahman H
 
Subject Brucellosis outbreak
Competitive enzyme-linked immunoassay
Indirect enzyme-linked immunoassay
Lateral flow assay
Rose bengal plate test
 
Description Not Available
Aim: The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized
buffalo farm.
Materials and Methods: A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA
and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay
(iELISA), and competitive ELISA (cELISA). The performances of LFA and other serological tests were evaluated using
OIE complaint cELISA as the gold standard.
Results: Serological tests revealed 50% of the animals were seropositive for Brucella antibodies and correlated with clinical
history of abortions, infertility, and productive failures. The newly developed assay showed 87.1% and 92.6% sensitivity
and specificity, which was even higher than the specificity of RBPT.
Conclusions: The investigation proved the potential usefulness of LFA for field diagnosis of brucellosis in the regions
where laboratory facilities are limited.
Not Available
 
Date 2018-11-05T09:08:26Z
2018-11-05T09:08:26Z
2015-04-01
 
Type Research Paper
 
Identifier Not Available
2231-0916
http://krishi.icar.gov.in/jspui/handle/123456789/9334
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World