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Serodiagnosis of animal trypanosomosis using a recombinant invariant surface glycoprotein of Trypanosoma evansi

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Title Serodiagnosis of animal trypanosomosis using a recombinant invariant surface glycoprotein of Trypanosoma evansi
Not Available
 
Creator Rudramurthy G R
Sengupta P P
Ligi M
Balamurugan V
Suresh K P
Rahman H
 
Subject Immunoreactivity
rISG-75ED
ROC analysis
Surra
 
Description Not Available
Trypanosomosis is endemic in many parts of the world in animals and humans. The diagnosis of asymptomatic carrier animals and a followup treatment is essential for control of the disease. In the present study, the extracellular domain (ED) region of invariant surface glycoprotein (ISG-75) gene of Trypanosoma evansi consisting of 1320 nucleotides (nt), encoding a polypeptide of 440 amino acids (aa) has been cloned and expressed in eukaryotic system Pichia pastoris (X-33). Immunoreactivity of the expressed protein (~70 kDa) was checked using a panel of standard serum samples. Furthermore, the diagnostic potential of the protein was evaluated using bovine, camel and horse serum samples from the field. Statistical analysis of the bovine data showed optimum combination of sensitivity and specificity at 98.6 and 99.7, respectively (>0.547 cut off OD value). The present finding revealed that the recombinant protein can be exploited as a potential diagnostic antigen in the serodiagnosis of trypanosomosis.
Not Available
 
Date 2018-11-12T05:01:15Z
2018-11-12T05:01:15Z
2017-04-01
 
Type Research Paper
 
Identifier Not Available
0975-1009
http://krishi.icar.gov.in/jspui/handle/123456789/10251
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Experimental Biology