Molecular characterization of MHC-DRB gene exon 2 in riverine buffalo (Bubalus bubalis)
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Molecular characterization of MHC-DRB gene exon 2 in riverine buffalo (Bubalus bubalis)
Not Available |
|
Creator |
S Naskar, S M Deb, S K Niranjan, D Sakaram, D Sharma, S Kumar, A Sharma
|
|
Subject |
Buffalo, DRB, MHC, PCR-RFLP, phylogeny
|
|
Description |
Not Available
In the present study, expressed DRB gene of buffalo major histocompatibility complex (Bubu) was characterized. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of Bubu-DRB second exon region using TaqI and HaeIII restriction enzymes revealed one and three restriction patterns, respectively. Comparative analysis of two different HaeIII variants revealed large number of amino acids substitutions at peptide binding site (PBS) of the molecule. The variants showed low divergence with cattle (BoLA) DRB3 alleles in general. Phylogenetically, Bubu-DRB clone b and e were found to be closer to Bubu-DRB*0501 and DRB*0401 alleles, respectively. High genetic variation at PBS region of Bubii-DRB exon 2 might contribute positively to antigen repertoire properties during infection. Not Available |
|
Date |
2019-12-04T05:50:35Z
2019-12-04T05:50:35Z 2014-10-01 |
|
Type |
Article
|
|
Identifier |
Not Available
0975-0967 http://krishi.icar.gov.in/jspui/handle/123456789/26880 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
CSIR-NISCAIR
|
|