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Molecular characterization of MHC-DRB gene exon 2 in riverine buffalo (Bubalus bubalis)

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Title Molecular characterization of MHC-DRB gene exon 2 in riverine buffalo (Bubalus bubalis)
Not Available
 
Creator S Naskar, S M Deb, S K Niranjan, D Sakaram, D Sharma, S Kumar, A Sharma
 
Subject Buffalo, DRB, MHC, PCR-RFLP, phylogeny
 
Description Not Available
In the present study, expressed DRB gene of buffalo major histocompatibility complex (Bubu) was characterized. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of Bubu-DRB second exon region using TaqI and HaeIII restriction enzymes revealed one and three restriction patterns, respectively. Comparative analysis of two different HaeIII variants revealed large number of amino acids substitutions at peptide binding site (PBS) of the molecule. The variants showed low divergence with cattle (BoLA) DRB3 alleles in general. Phylogenetically, Bubu-DRB clone b and e were found to be closer to Bubu-DRB*0501 and DRB*0401 alleles, respectively. High genetic variation at PBS region of Bubii-DRB exon 2 might contribute positively to antigen repertoire properties during infection.
Not Available
 
Date 2019-12-04T05:50:35Z
2019-12-04T05:50:35Z
2014-10-01
 
Type Article
 
Identifier Not Available
0975-0967
http://krishi.icar.gov.in/jspui/handle/123456789/26880
 
Language English
 
Relation Not Available;
 
Publisher CSIR-NISCAIR