Development of naive phage display VHH libraries from Indian camel
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Development of naive phage display VHH libraries from Indian camel
Not Available |
|
Creator |
Lipsa Dash
Saravanan Subramaniam S.A. Khulape B.R. Prusty Kamal Pargai Shirish Dadarao Narnaware Niteen V Patil B. Pattnaik |
|
Subject |
Nano body
phagemid Redundancy Single pot library |
|
Description |
Not Available
The present study describes the construction of naïve camelid VHH single pot library from lymphocytes of non-immunized Camelus dromedarius. RNA extracted from 6 healthy female camels was pooled together and used for construction of library in phagemid vector. The VHH inserts were found in 50-70% of the phagemid vector (pMECS vector) cloned in Escherichia coli and the size of the libraries was estimated to be around 10⁵-10⁷cfu. Sequence analysis of VHHs revealed variations in the length of complementary determining regions and amino acid usage resulting in a variety of different paratopes. Further expression of VHH rescued from the library was optimized in prokaryotic system using pET32 expression system. Functional antibodies generated from this naïve library will be explored for affinity against various antigens in further studies. © 2016, Indian Council of Agricultural Research. All rights reserved. Not Available |
|
Date |
2020-02-12T05:06:03Z
2020-02-12T05:06:03Z 2016-01-01 |
|
Type |
Article
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/31819 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Not Available
|
|