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Chimeric foot-and-mouth disease virus serotype O displaying a serotype Asia1 antigenic epitope at the surface

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Title Chimeric foot-and-mouth disease virus serotype O displaying a serotype Asia1 antigenic epitope at the surface
 
Creator Jitendra K Biswal
Rajeev Ranjan
Bramhadev Pattnaik
 
Subject Asia1 epitope; Chimeric foot-and-mouth disease virus; Epitope insertion; Foot-and-mouth disease virus; Reverse genetics
 
Description Objective: To determine whether the G-H loop of foot-and-mouth disease virus (FMDV) serotype O can function as a target structure to harbour and display serotype Asia1 antigenic epitope at the surface.

Results: Using reverse genetics, FMDV serotype O IND R2/1975 displaying a FMDV serotype Asia1 B cell epitope at the capsid surface was constructed. The epitope-inserted recombinant chimeric virus was genetically stable up to ten serial passages in cell culture and exhibited growth properties similar to the parental serotype O virus. Furthermore, the surface-displayed Asia1 epitope able to react with serotype Asia1 specific antibodies in a competitive ELISA. Importantly, the recombinant chimeric virus showed neutralizing activity to both serotype O and Asia1 polyclonal antibodies.

Conclusion: The capsid protein of FMDV serotype O can effectively display potent epitope of other serotypes, making this an attractive approach for the design of new generation bi-valent FMD vaccines.
 
Date 2021-07-30T05:37:59Z
2021-07-30T05:37:59Z
2016-09-01
 
Type Journal
 
Identifier Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/51392
 
Language English
 
Relation Not Available;
 
Publisher Springer