Effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive Indian River buffaloes (Bubalus bubalis).
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive Indian River buffaloes (Bubalus bubalis).
Not Available |
|
Creator |
Manjunatha BM
Ravindra JP Gupta PSP Devaraj M Nandi S |
|
Subject |
OPU
Buffalo Oocyte Embryo Season |
|
Description |
Not Available
The present study was carried out to examine the effect of season on in vivo oocyte recovery and embryo production in non-descriptive, Indian river buffaloes (Bubalus bubalis). Ovum pick up (OPU) was conducted twice a week for 8 weeks during peak (October–March) and low (April–September) breeding season in live buffaloes (n = 6). OPU was performed using ultrasound equipment with a 5 MHz transvaginal transducer, a single lumen 18-gauge, 55-cm long needle and a constant vacuum pressure of 110 mmHg. The number and size of follicles was determined before puncture. The recovered oocytes were graded and only grade A and grade B oocytes were used for in vitro production (IVP) of embryos. The mean number of follicles observed per animal per session did not differed (P < 0.05) between animals or between puncture sessions in both low and peak breeding seasons. Higher (P < 0.05) number of follicles were observed (4.8 ± 0.2 versus 3.1 ± 0.3) and punctured (4.0 ± 0.2 versus 2.4 ± 0.2) during peak breeding season when compared to low breeding season. Oocytes recovered (1.6 ± 0.1 versus 1.0 ± 0.3) per animal per session were higher (P < 0.05) in peak breeding season than low breeding season. During the peak breeding season, the blastocyst yield per animal per session (0.3 ± 0.4 versus 0.18 ± 0.4) was higher (P < 0.05) than the low breeding season. However, season did not significantly affect the percentage of oocytes suitable for IVP (grade A + B) and blastocyst production rate. In conclusion, the efficiency of OPU combined with IVP was higher during the peak breeding season than the low breeding season in buffaloes. Not Available |
|
Date |
2021-08-05T03:23:21Z
2021-08-05T03:23:21Z 2009-01-01 |
|
Type |
Journal
|
|
Identifier |
Manjunatha BM, Ravindra JP, Gupta PSP, Devaraj M and Nandi S (2009) Effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive Indian River buffaloes (Bubalus bubalis). Animal Reproduction Science. 111:376-383.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/53133 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Elsevier Ltd
|
|