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Lateral flow assay based rapid detection of cephalexin in milk.

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Title Lateral flow assay based rapid detection of cephalexin in milk.
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Creator Lata, K.; Sharma, R.; Naik, L.; Rajput, Y.S. and Mann, B.
 
Subject CEPHALEXIN, MILK
 
Description Not Available
A rapid and semiquantitative lateral flow assay has been developed for detection of
cephalexin (CFX) in milk. The assay is based on a competitive format using
polyclonal antibodies. Antibodies were raised in rabbits against CFX-keyhole
limpet hemocyanin. The specificity of antibodies was ascertained by direct
enzyme-linked immunosorbent assay. The laboratory prepared gold nanoparticles
coupled to anti-CFX antibodies, which competed for free CFX in sample and
CFX–bovine serum albumin conjugate on the test line of a lateral flow strip. The
control line of the lateral flow strip consisted of species-specific antibody. The
assay was validated with spiked milk samples and involved application of skim
milk to the adsorbent pad followed by visualization of red color on test and
control lines within 8 min. The lower detection limit of CFX was 30 ppb in milk,
which is lower than the Codex prescribed maximum residue limit. The storage
stability of the prepared strip was also established
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Date 2021-08-26T08:31:37Z
2021-08-26T08:31:37Z
2015-06-28
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/60753
 
Language English
 
Relation Not Available;
 
Publisher Not Available