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Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells

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Title Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells
Not Available
 
Creator Not AvailableJaya Bharati, Satish Kumar, Sunil Kumar, NH Mohan, Rafiqul Islam, Seema Rani Pegu, Santanu Banik, Bikash Chandra Das, Sanjib Borah, Mihir Sarkar
 
Subject Androgen receptor; steroidogenesis; proliferation; viability; luteal cell; porcine
 
Description Not Available
Luteal steroidogenesis is critical to implantation and pregnancy maintenance in mammals.
The role of androgen receptors (AR) in the progesterone (P4) producing luteal cells of porcine corpus luteum (CL) remains unexplored. The aim of the present study was to establish
AR gene knock out (KO) porcine luteal cell culture system model by CRISPR/Cas9 genome
editing technology and to study the downstream effects of AR gene deficiency on steroidogenic potential and viability of luteal cells. For this purpose, genomic cleavage detection
assay, microscopy, RT-qPCR, ELISA, annexin, MTT, and viability assay complemented by bioinformatics analysis were employed. There was significant downregulation (p < 0.05) in the
relative mRNA expression of steroidogenic marker genes STAR, CYP11A1, HSD3B1 in AR KO
luteal cells as compared to the control group, which was further validated by the significant
(p < 0.05) decrease in the P4 production. Significant decrease (p < 0.05) in relative viability
on third passage were also observed. The relative mRNA expression of hypoxia related gene
HIF1A was significantly (p < 0.05) downregulated in AR KO luteal cells. Protein-protein interaction analysis mapped AR to signaling pathways associated with luteal cell functionality.
These findings suggests that AR gene functionality is critical to luteal cell steroidogenesis
in porcine.
Not Available
 
Date 2022-09-24T05:22:03Z
2022-09-24T05:22:03Z
2022-06-08
 
Type Research Paper
 
Identifier Bharati, Jaya, Satish Kumar, Sunil Kumar, N. H. Mohan, Rafiqul Islam, Seema Rani Pegu, Santanu Banik, Bikash Chandra Das, Sanjib Borah, and Mihir Sarkar. "Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells." Animal Biotechnology (2022): 1-14.
http://krishi.icar.gov.in/jspui/handle/123456789/74560
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis