Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells
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Title |
Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells
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Creator |
Not AvailableJaya Bharati, Satish Kumar, Sunil Kumar, NH Mohan, Rafiqul Islam, Seema Rani Pegu, Santanu Banik, Bikash Chandra Das, Sanjib Borah, Mihir Sarkar
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Subject |
Androgen receptor; steroidogenesis; proliferation; viability; luteal cell; porcine
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Description |
Not Available
Luteal steroidogenesis is critical to implantation and pregnancy maintenance in mammals. The role of androgen receptors (AR) in the progesterone (P4) producing luteal cells of porcine corpus luteum (CL) remains unexplored. The aim of the present study was to establish AR gene knock out (KO) porcine luteal cell culture system model by CRISPR/Cas9 genome editing technology and to study the downstream effects of AR gene deficiency on steroidogenic potential and viability of luteal cells. For this purpose, genomic cleavage detection assay, microscopy, RT-qPCR, ELISA, annexin, MTT, and viability assay complemented by bioinformatics analysis were employed. There was significant downregulation (p < 0.05) in the relative mRNA expression of steroidogenic marker genes STAR, CYP11A1, HSD3B1 in AR KO luteal cells as compared to the control group, which was further validated by the significant (p < 0.05) decrease in the P4 production. Significant decrease (p < 0.05) in relative viability on third passage were also observed. The relative mRNA expression of hypoxia related gene HIF1A was significantly (p < 0.05) downregulated in AR KO luteal cells. Protein-protein interaction analysis mapped AR to signaling pathways associated with luteal cell functionality. These findings suggests that AR gene functionality is critical to luteal cell steroidogenesis in porcine. Not Available |
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Date |
2022-09-24T05:22:03Z
2022-09-24T05:22:03Z 2022-06-08 |
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Type |
Research Paper
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Identifier |
Bharati, Jaya, Satish Kumar, Sunil Kumar, N. H. Mohan, Rafiqul Islam, Seema Rani Pegu, Santanu Banik, Bikash Chandra Das, Sanjib Borah, and Mihir Sarkar. "Androgen receptor gene deficiency results in the reduction of steroidogenic potential in porcine luteal cells." Animal Biotechnology (2022): 1-14.
http://krishi.icar.gov.in/jspui/handle/123456789/74560 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Taylor and Francis
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