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Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing

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Title Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing
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Creator Anand joseph
Indu Baiju
Irfan A. Bhat
Sriti Pandey
Mukesh Bharti
Megha Verma
Anuj Pratap Singh
Matin M. Ansari
Vikash Chandra
Gutulla Saikumar
Amarpal
Gutulla Taru Sharma
 
Subject conditioned media
growth factors
stem cells
wound healing
 
Description Not Available
Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone marrow‐derived MSCs‐CM in excision wound healing in a guinea pig model. MSCs were obtained from bone marrow, expanded ex vivo and characterized as per ISCT criteria. CM was collected assayed by western blot to ascertain the presence of important secretory biomolecules. Quantitative estimation by enzyme‐linked immunosorbent assay was done for a vascular epidermal growth factor (VEGF) and interleukin‐6 (IL‐6) in caprine MSCs‐CM and optimum time for collection of CM was decided as 72 hr. CM from all the species was lyophilized by freeze‐drying method. Full‐thickness (2 × 2 cm2) excision skin wounds were created in guinea pigs (six animals in each group) and respective lyophilized CM mixed with laminin gel was applied topically at weekly interval. On Day 28, histopathological examinations of healed skin were done by hemotoxylin and eosin staining. MSCs were found to secrete important growth factors and cytokines (i.e., VEGF, transforming growth factor‐β1, fibroblast growth factor‐2, insulin‐like growth factor‐1, stem cell factor, and IL‐6) as demonstrated by immunohistochemistry and western blot assay. It was found that allogenic and xenogenic application of CM significantly improved quality wound healing with minimal scar formation. Thus, MSCs‐CM can be used allogenically as well as xenogenically for quality wound healing.
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Date 2021-04-30T06:37:44Z
2021-04-30T06:37:44Z
2020-01-21
 
Type Article
 
Identifier Not Available
0021-9541
http://krishi.icar.gov.in/jspui/handle/123456789/46910
 
Language English
 
Relation Not Available;
 
Publisher wiley