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Rapid polymerase chain reaction (PCR) for detection of pathogenic leptospires in biological samples

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Title Rapid polymerase chain reaction (PCR) for detection of pathogenic leptospires in biological samples
Not Available
 
Creator Bhure SK
Lakshmisha KV
Patil SS
Shome BR
Shome R
Gangadhar NL
Gajendragad MR
Prabhudas K
 
Subject Leptospirosis
Lip L32 gene
Rapid PCR
 
Description Not Available
Leptospirosis, considered to be the most widespread zoonoses in the world, is caused by serovars belonging to 7 pathogenic species of the genus Leptospira, viz. L. borgpetersenii, L. inadai, L. interrogans, L. kirschneri, L. noguchii, L. santarosai, and L. weilii (Ramadass et al. 1992). This paper reports the development of molecular technique for detecion of pathogenic Leptospira species by PCR amplification of a partial fragment of the major outer- membrane lipoprotein LipL32 gene, whose protein appears to be an important virulence factor (Haake et al. 2000, Yang et al. 2002), confined to pathogenic strains of all Leptospira spp. The technique could detect majority of serovars of L. interrogans, viz. icterohaemorrhagiae, canicola, autumnalis, javonica, pomona, pyrogenes, australis; L. borgpetersenii serovar Hardjobovis, and L. inadai.
Not Available
 
Date 2021-08-16T08:41:04Z
2021-08-16T08:41:04Z
2009-08-10
 
Type Research Paper
 
Identifier Bhure SK, Lakshmisha KV, Patil SS, Shome BR, Shome R, Gangadhar NL, Gajendragad MR and Prabhudas K. 2009.Rapid polymerase chain reaction (PCR) for detection of pathogenic leptospires in biological samples.Indian Journal of Animal Sciences 79(8): 781–783
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/57237
 
Language English
 
Relation Not Available;
 
Publisher Indian Journal of Animal Sciences