Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on tricine SDS-PAGE gels.
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on tricine SDS-PAGE gels.
Not Available |
|
Creator |
Not Available
Sharma, N.; Sharma, R.; Rajput, Y. S.; Mann, B., Gandhi, K. |
|
Subject |
Lateral flow Assay
|
|
Description |
Not Available
A competitive lateral flow immunoassay using amorphous carbon nanoparticles (CNPs) and nonimmunoglobulin antigen has been developed for the rapid detection of adulteration of cow’s milk with buffalo’s milk. Purified polyclonal antibodies against a specific buffalo’s milk protein fraction were conjugated to CNPs and sprayed on a conjugate pad. The test line consisted of buffalo’s skimmed milk proteins (1.6 μg/cm), while the control line contained anti-rabbit antibodies raised in goat (0.5 μg/cm). In the test procedure milk sample is mixed with 100 mM borate buffer (pH 8.8 containing 1% BSA and 0.05% Tween 20) and pipetted onto the sample-cum-conjugate pad. A black/grey test line can be observed if the sample is free from buffalo’s milk. The sensitivity of the test i.e. no visible test line is 5% adulteration of cow’s milk with buffalo’s milk. The test has applicability at the milk receiving stations and can be applied to heated milk samples. Not Available |
|
Date |
2021-08-26T08:24:00Z
2021-08-26T08:24:00Z 2021-02-20 |
|
Type |
Article
|
|
Identifier |
Sharma, N.; Sharma, R.; Rajput, Y. S.; Mann, B., Gandhi, K. (2021). Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on tricine SDS-PAGE gels. Food Chemistry. 340, 127923, ISSN 0308-8146, https://doi.org/10.1016/j.foodchem.2020.127923 .
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60702 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Elsevier
|
|