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Rapid screening test for detection of oxytetracycline residues in milk using lateral flow assay

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Title Rapid screening test for detection of oxytetracycline residues in milk using lateral flow assay
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Creator Naik, L., Sharma, R., Mann, B., Lata, K., Rajput, Y.S. and Nath, B.S.,
 
Subject Milk Oxytetracycline Lateral flow assay Gold nanoparticles Antibiotics
 
Description Not Available
A rapid, semi-quantitative lateral flow assay (LFA) was developed to screen the oxytetracycline (OTC)
antibiotics residues in milk samples. In this study a competitive immuno-assay format was established.
Colloidal gold nano-particles (GNP) were prepared and used as labelling material in LFA. Polyclonal anti bodies were generated against OTC molecule (anti-OTC), purified and the quality was assessed by enzyme
linked immuno sorbet assay. For the first time membrane components required for LFA in milk system
was optimized. GNP and anti-OTC stable conjugate preparation method was standardized, and then these
components were placed over the conjugate pad. OTC coupled with carrier protein was placed on test
line; species specific secondary antibodies were placed on the control line of the membrane matrix.
Assay was validated by spiking OTC to antibiotic free milk samples and results could be accomplished
within 5 min. without need of any equipment. The visual detection limit was 30 ppb.
Not Available
 
Date 2021-08-26T08:32:25Z
2021-08-26T08:32:25Z
1001-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/60760
 
Language English
 
Relation Not Available;
 
Publisher Not Available