ICAR Research Data Repository for Knowledge Management
Rapid enzymatic assay for lactate content in milk – an indirect method to ascertain the presence of neutralizers in microbial deteriorated milk.
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View Archive Info| Field | Value | |
| Title |
Rapid enzymatic assay for lactate content in milk – an indirect method to ascertain the presence of neutralizers in microbial deteriorated milk.
Not Available |
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| Creator |
Kumar, B.; Sharma, R.; Thakur, R.; Barui, A.K.; Rajput, Y.S. and Mann, B.
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| Subject |
lactate, lactate oxidase
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| Description |
Not Available
A rapid method for qualitative and quantitative assaying of l-lactate in milk is described. The method is based on oxidation of lactate by lactate oxidase with the simultaneous formation of H2O2 which subsequently oxidises 2, 4, 6-tribromo 3-hydroxy benzoic acid (TBHBA) in the presence of peroxidase. Oxidised TBHBA then reacts with 4-amino antipyrine to obtain magenta colour (λmax = 523 nm). In the qualitative assay, colour change becomes visible in raw milk samples spiked with 300 mg lactate/L. The absorbance increases linearly up to 1000 mg/L. The method can be used for rapid assessment of the hygienic quality of milk. Not Available |
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| Date |
2021-08-26T08:31:44Z
2021-08-26T08:31:44Z 2016-02-16 |
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| Type |
Research Paper
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/60754 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
Not Available
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